vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-305Research ArticleСтатьиАНАЛИЗ ВАРИАБЕЛЬНОСТИ МИКРОСАТЕЛЛИТНЫХ ЛОКУСОВ У ПРЕДСТАВИТЕЛЕЙ РОДА СМОРОДИНЫ (RIBES L.), ВЫРАЩИВАЕМЫХ В БЕЛАРУСИANALYSIS OF MICROSATELLITE LOCI VARIABILITY RIBES L. REPRESENTATIVES GROWN IN BELARUSМежнинаО. А.MezhninaO. A.

мл. науч. сотрудник

Junior researcher

olgamezhnina@gmail.comУрбановичО. Ю.UrbanovichO. Yo.

д-р биол. наук, заведующий лабораторией

D. Sc. (Biol.), Head of the Laboratory

O.Urbanovich@igc.byИнститут генетики и цитологии НАН БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus201705082017034554Copyright © Межнина О.А., Урбанович О.Ю., 20172017Межнина О.А., Урбанович О.Ю.Mezhnina O.A., Urbanovich O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/305

С помощью 8 SSR-маркеров изучено генетическое разнообразие 86 представителей рода Ribes, выращиваемых в Республике Беларусь. Показано, что сорта смородины черной, смородины красной и крыжовника обыкновенного, выращиваемые в Республике Беларусь, характеризуются достаточно высоким разнообразием аллелей локусов микросателлитных последовательностей. Среднее количество аллелей на локус среди 86 образцов составило 12,1, среднее количество уникальных генотипов в расчете на маркер – 26,4. Значение дискриминационной силы для всех маркеров высокое (в среднем – 0,8). Образцы, относящиеся к разным видам, формируют отдельные кластеры на дендрограмме филогенетического сходства. Генетически наиболее близки смородине черной йошта, смородина золотистая, смородина альпийская. На большем генетическом расстоянии находится смородина красная. Сорта крыжовника формируют кластер, наиболее отдаленный от видов смородины. Анализ распределения аллелей показал, что сорта смородины красной и крыжовника обыкновенного имеют отличные от сортов смородины черной SSR-аллели по исследованным локусам микросателлитных последовательностей. Число аллелей, встречавшихся только у сортов смородины красной и крыжовника обыкновенного, варьировалось от 1 (локусы g2-G12, g1-M0) до 5 (локус E4D03). На основании анализа полиморфизма SSR-локусов сформирован набор из 8 маркеров, позволяющий проводить ДНК-идентификацию генотипов представителей рода Ribes. При выборе набора учтен уровень информативности каждого маркера, частота встречаемости аллелей, выявляемых данным набором маркеров у сортов, а также удобство визуализации и анализа продуктов амплификации. Метод SSR-анализа с использованием указанного набора маркеров может успешно применяться для идентификации смородины черной, смородины красной и крыжовника обыкновенного на молекулярном уровне. 

The study of the genetic diversity of 86 representatives of the genus Ribes cultivated in Belarus using 8 SSR markers was held. It is shown that the varieties of black currant, red currant and gooseberry grown in Belarus, is characterized by high diversity of microsatellite loci. The average number of alleles per locus among the 86 samples was 12.1. The average number of unique genotypes per marker was 26.4. The value of discriminatory power for all markers was high with average number 0.8. Samples belonging to different species formed distinct clusters in the dendrogram of phylogenetic similarity. Genetically closest to black currant were josta, Golden currant, Alpine currant. At a greater genetic distance were red currants. Varieties of gooseberries form a cluster that is most distant from currant species. Analysis of alleles distribution at the investigated loci of microsatellite sequences revealed that red currant and gooseberry varieties have different SSR-alleles from black currant varieties. The number of alleles detected only for red currant and gooseberry varieties ranged from 1 (loci g2-G12, g1-M0) to 5 (locus E4D03). Based on the analysis of SSR-locus polymorphism, a set of 8 markers was generated that allow DNA-identification of Ribes genotypes. For selection the set of markers the level of information value for each marker, the frequency of alleles occurrence and convenience of visualization and analysis of amplification products were taken into account. The method of SSR-analysis with using specified set of markers can be successfully applied for identification of black currant, red currant and gooseberry varieties at the molecular level.

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The authors declare that there are no conflicts of interest present.