vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-290Research ArticleСтатьиМОЛЕКУЛЯРНАЯ ХАРАКТЕРИСТИКА ИЗОЛЯТА ВИРУСА КРАСНОЙ КОЛЬЦЕВОЙ ПЯТНИСТОСТИ ГОЛУБИКИMOLECULAR CHARACTERIZATION OF BLUEBERRY RED RINGSPOT VIRUS ISOLATEБожидайТ. Н.BazhydaiT. N.

науч. сотрудник

Scientific researcher

tanya_bozhidaj@mail.ruКухарчикН. В.KukharchykN. V.

д-р с.-х. наук, доцент, заведующий отделом

D. Sc. (Agricult.), Assistant Professor, Head of the Department

kychnataly@rambler.ruИнститут плодоводстваInstitute for Fruit Growing201704082017022732Copyright © Божидай Т.Н., Кухарчик Н.В., 20172017Божидай Т.Н., Кухарчик Н.В.Bazhydai T.N., Kukharchyk N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/290

Вирус красной кольцевой пятнистости голубики (BRRV) является одним из вредоносных вирусов брусничных культур и, в соответствии с нормативными документами Европейской и Средиземноморской организации по защите растений (EPPO), подлежит контролю и не допускается при производстве сертифицированного посадочного материала Vaccinium spp. Отсутствие данных о белорусских изолятах BRRV определило необходимость проведения детального анализа данного вируса с использованием PCR-диагностики, секвенирования и анализа нуклеотидных последовательностей вируса. В результате впервые охарактеризован на молекулярном уровне изолят BRRV из Беларуси (BRRV-BY1). В ходе работы амплифицирован и секвенирован фрагмент гена активатора транскрипции вируса. Полученная нуклеотидная последовательность помещена в международную базу данных (EMBL/GenBank), где ей присвоен идентификационный номер LN998983. Филогенетический анализ последовательностей участка генома BRRV показал, что белорусский изолят наиболее родственен изолятам вируса из США. Корреляции между кластерированием изолятов и их географическим происхождением не обнаружено. 

The fragment of BRRV isolate from Belarus (BRRV-BY1) was sequenced for the first time and nucleotide sequence of this isolate was deposited in GenBank with the accession No. LN998983. Sequence analysis revealed that the Belarusian isolate shared 94.1–99.3 % identity with 18 isolates of the virus available in GenBank. BRRV isolate from Belarus was mostly closely related to those from the USA (accession Nos JF917083 and JF917082) as they shared 99.3 % nucleotide identity. Phylogenetic analysis showed that clustering of investigated isolates didn’t depend on their geographical origin.

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The authors declare that there are no conflicts of interest present.