vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-288Research ArticleСтатьиПРОЛИФЕРАТИВНАЯ АКТИВНОСТЬ И МЕМБРАННЫЙ ПОТЕНЦИАЛ КЛЕТОК ЛИНИЙ С6 И HELA ПРИ КУЛЬТИВИРОВАНИИ В УСЛОВИЯХ ЭЛЕКТРИЧЕСКОЙ СТИМУЛЯЦИИPROLIFERATIVE ACTIVITY AND MEMBRANE POTENTIAL OF C6 AND HELA CELL LINES IN CULTURE UNDER ELECTRICAL STIMULATIONКуницкаяЮ. Н.KunitskayaY.

аспирант

yuliya.kunitskaya@gmail.comКочетковаТ. А.KochetkovaT.

студент

kochetkovatan@gmail.comКоваленкоЕ. А.KavalenkaE.

студент

kovalenko.elizabeth@gmail.comГолубеваЕ. Н.GolubevaE.

старший преподаватель

golubeva.ln.87@gmail.comБулайП. М.BulaiP.

канд. физ.-мат. наук, доцент

bulaypm@bsu.byМолчановП. Г.MolchanovP.

науч. сотрудник

pavel.g.molchanov@gmail.comДенисовА. А.DenisovA.

канд. биол. наук, заведующий лабораторией

an.denisov@gmail.comПитликТ. Н.PitlikT.

канд. биол. наук, доцент

taras.pitlik@gmail.comЧеренкевичС. Н.CherenkevichS.

академик, д-р биол. наук, профессор, заведующий кафедрой

cherenkevich@bsu.byБелорусский государственный университетBelarusian State University20170208201702713Copyright © Куницкая Ю.Н., Кочеткова Т.А., Коваленко Е.А., Голубева Е.Н., Булай П.М., Молчанов П.Г., Денисов А.А., Питлик Т.Н., Черенкевич С.Н., 20172017Куницкая Ю.Н., Кочеткова Т.А., Коваленко Е.А., Голубева Е.Н., Булай П.М., Молчанов П.Г., Денисов А.А., Питлик Т.Н., Черенкевич С.Н.Kunitskaya Y., Kochetkova T., Kavalenka E., Golubeva E., Bulai P., Molchanov P., Denisov A., Pitlik T., Cherenkevich S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/288

Исследовано влияние долговременной электрической стимуляции на функциональные свойства клеток в культуре. В работе использованы клетки глиомы крысы линии С6 и клетки аденокарциномы шейки матки линии HeLa. Стимуляцию проводили на протяжении 12 ч однородным электрическим полем с частотой 10 Гц. Для измерения величины равновесного трансмембранного потенциала применяли метод пэтч-кламп, пролиферативную активность оценивали по изменению числа клеток относительно их числа в контрольном образце. Показано, что электрическая стимуляция вызывает изменение равновесного трансмембранного потенциала клеток, что приводит к последующему изменению их пролиферативной активности. Выявлено, что деполяризация плазматической мембраны усиливает пролиферативную активность клеток, в то время как гиперполяризация замедляет. Установлено, что конечный результат зависит как от параметров электрического поля, так и от типа клеток. Таким образом, наблюдаемый эффект связан с активацией потенциал-зависимых ионных каналов, приводящей к изменению времени прохождения клеткой определенной фазы клеточного цикла. Полученные результаты могут быть использованы для разработки методов клеточной инженерии. 

The influence of long-term electrical stimulation on the functional properties of cells in culture was investigated. C6 rat glioma cells and HeLa human adenocarcinoma cells were used in this work. Stimulation was carried out with uniform electric field for 12 hours with the train of pulses at a frequency of 10 Hz. Measurements of membrane potential were conducted using patch-clamp technique; the proliferative activity was evaluated by cell counting relative to control sample. It was shown that electrical stimulation causes a change in membrane potential of the cells and subsequent changes in their proliferative activity. It was established that membrane depolarisation increases proliferative activity whereas hyperpolarization decreases it. It was established that the effect depends on the parameters of the electric field and the type of cells. The observed effect is associated with activation of potential-dependent ion channels, resulting in changes of the specific phase duration in the cell cycle. The obtained results can be used in development methods of cell engineering.

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The authors declare that there are no conflicts of interest present.