vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-273Research ArticleСтатьиСОЗДАНИЕ ТРАНСГЕННЫХ РАСТЕНИЙ NICOTIANA TABACUM С ГЕНОМ NDB2 ARABIDOPSIS THALIANA ДЛЯ ИЗУЧЕНИЯ ОТВЕТА НА СТРЕССTHE CREATION OF TRANSGENIC PLANTS NICOTIANA TABACUM WITH GENE NDB2 ARABIDOPSIS THALIANA TO STUDY RESPONSE TO STRESSСавчинД. В.SauchynD. V.

канд. биол. наук, науч. сотрудник

Ph. D. (Biol.), Researcher

sauchyndzmitry@gmail.comКузмицкаяП. В.KuzmitskayaP. V.

науч. сотрудник

Researcher

1401polina@gmail.comУрбановичО. Ю.UrbanovichO. Yu.

д-р биол. наук, заведующий лабораторией

D. Sc. (Biol.), the Head of the laboratory

O.Urbanovich@igc.byФедосееваИ. В.BorovskiiG. B.

канд. биол. наук, науч. сотрудник

Ph. D. (Biol.), Researcher

borovskii@sifibr.irk.ruБоровскийГ. Б.FedoseevaI. V.

д-р биол. наук, профессор, гл. науч. сотрудник

D. Sc. (Biol.), Professor, Chief researcher

borovskii@sifibr.irk.ruИнститут генетики и цитологии НАН БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusСибирский институт физиологии и биохимии растений Сибирского отделения РАНSiberian Institute of Plant Physiology and Biochemistry Siberian Branch of Russian Academy of Sciences201718022017015461Copyright © Савчин Д.В., Кузмицкая П.В., Урбанович О.Ю., Федосеева И.В., Боровский Г.Б., 20172017Савчин Д.В., Кузмицкая П.В., Урбанович О.Ю., Федосеева И.В., Боровский Г.Б.Sauchyn D.V., Kuzmitskaya P.V., Urbanovich O.Y., Borovskii G.B., Fedoseeva I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/273

С целью изучения влияния измененного уровня экспрессии гена ndb2 (NAD(P)H-дегидрогеназа B2, КФ 1.6.5.2) на активность других белков митохондриальной локализации и устойчивость растений к различным стрессам созданы трансгенные растения табака, экспрессирующие данный ген. Ген ndb2 длиной 1749 п.н. выделен из суммарной матричной РНК Arabidopsis thaliana с помощью ОТ-ПЦР. Данный ген клонирован в плазмиду pBI121. На основе плазмиды pBI121 создана векторная конструкция pBI121_NDB2, содержащая ген ndb2 под контролем 35S CaMV промотора. Показана эффективность pBI121_NDB2 для трансформации. Экспериментально подтверждены факты интеграции гена ndb2 в геноме.

 

Transgenic tobacco plants that express the ndb2 gene were obtained to study the influence of changed level of the ndb2 gene (NAD(P)H dehydrogenase B2, EC 1.6.5.2) expression on the activity of the proteins of mitochondrial localization and stress resistance of plants. The ndb2 gene of 1749 bp was isolated from Arabidopsis thaliana total messenger RNA using RT-PCR. This gene was cloned into the pBI121 plasmid. The pBI121 _ NDB2 vector construction containing the ndb2 gene under the control of the 35S CaMV promoter was created on the basis of the pBI121 plasmid. The pBI121_NDB2 efficiency for transformation was demonstrated. The ndb2 gene integration into the tobacco genome was confirmed experimentally. The tobacco lines expressing target ndb2 gene were obtained.

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The authors declare that there are no conflicts of interest present.