vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-270Research ArticleСтатьиПОЛУЧЕНИЕ МУТАНТОВ CLOSTRIDIUM ACETOBUTYLICUM S1, УСТОЙЧИВЫХ К БУТАНОЛУSELECTION OF BUTANOL-RESISTANT MUTANTS OF CLOSTRIDIUM ACETOBUTYLICUM S1БолотникЕ. В.BolotnikE. V.

канд. биол. наук, заместитель директора

Ph. D. (Biol.), Deputy Director

bolotnik_allena@mbio.bas-net.byЧерешневА. А.ChereshnevA. A.

магистрант

Graduate student

chereshnev.biochem@gmail.comТитокМ. А.TitokM. A.

д-p биол. наук, профеcсор, гл. науч. сотрудник

D. Sc. (Biol.), Professor, Chief researcher

litvinovich@mbio.bas-net.byКоломиецЭ. И.KolomietsE. I.

чл.-кор., д-р биол. наук, генеральный директор Государственного научно-производственного объединения «Химический синтез и биотехнологии» – директор Института микробиологии НАН Беларуси

Corresponding Member, D. Sc. (Biol.), General Director of State Scientific-Production Association “Chemical Synthesis and Biotechnology” – Director of Institute of Microbiology of the National Academy of Sciences of Belarus

kolomiets@mbio.bas-net.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of BelarusБелорусский государственный университетBelarusian State UniversityГосударственное научно-производственное объединение «Химический синтез и биотехнологии» Институт микробиологии НАН БеларусиState Scientific-Production Association “Chemical Synthesis and Biotechnology” Institute of Microbiology of the National Academy of Sciences of Belarus201718022017013038Copyright © Болотник Е.В., Черешнев А.А., Титок М.А., Коломиец Э.И., 20172017Болотник Е.В., Черешнев А.А., Титок М.А., Коломиец Э.И.Bolotnik E.V., Chereshnev A.A., Titok M.A., Kolomiets E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/270

Оптимизированы условия химического мутагенеза бактерий C. acetobutylicum S1. Показано, что обработка бактериальной культуры, выращенной в течение 8 ч в стандартной синтетической среде MSS, N-метил-N′-нитро-N- нитрозогуанидином в концентрации 50 мкг/мл в цитратном буфере (рН = 5,5) в течение 60 мин при 25 ºС позволяет отбирать варианты мутантов, стабильно наследующих признак устойчивости к бутанолу в концентрации 2,5 %. Анализ состава жирных кислот цитоплазматической мембраны выявил наличие 2-гексилциклопропаноктановой кислоты у мутанта, характеризующегося повышенной продукцией бутанола.

 

Conditions were optimized for chemical mutagenesis of bacteria C. acetobutylicum S1. It was shown that treatment of bacterial culture pre-grown for 8 hours in standard synthetic MSS medium, with N-methyl-N′-nitro-N-nitrosoguanidine at concentration 50 μg/ml in citrate buffer (pH = 5.5) during 60 min at 25 °C allowed to select mutantvariants stably inheriting the trait of resistance to 2.5 % butanol. Analysis of fatty acid composition of cytoplasmic membrane has revealed presence of 2-hexylcyclopropane octanoic acid in mutant distinguished by elevated butanol production.

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The authors declare that there are no conflicts of interest present.