vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-255Research ArticleСтатьиМЕТОДОЛОГИЯ ИССЛЕДОВАНИЯ ДИНАМИКИ СИНТЕЗА И СЕКРЕЦИИ АНТИГЕНОВ НЕПАТОГЕННЫХ МИКРООРГАНИЗМОВ НА ПРИМЕРЕ LACTOCOCCUS LACTISMETHODOLOGICAL APPROACH TO THE STUDY OF THE DYNAMIC OF ANTIGENS SYNTHESIS AND SECRETION BY NONPATHOGENIC ORGANISMS ON THE EXAMPLE OF LACTOCOCCUS LACTISКиселеваЕ. П.KiselevaE.

канд. хим. наук, вед. науч. сотрудник

Ph. D. (Chem.), Liding Researcher

epkiseleva@yandex.ruЕзубецА. П.EzybetsA.

студент

Student

anna.ezubets94@gmail.comМихайлопулоК. И.MikhailopuloK.

науч. сотрудник

 

Researcher

k.mikhailopulo@gmail.comНовикГ. И.NovikG.

канд. биол. наук, зав. лабораторией «Коллекция микроорганизмов»

Ph. D. (Biol.), Head of the Laboratory “Collection of Microorganisms”

galina_novik@mbio.bas-net.byИнститут биоорганической химии НАН БеларусиБеларусьInstitute of Bioorganic Chemistry of the National Academy of Sciences of BelarusBelarusБелорусский государственный университетБеларусьByelarusian State UniversityBelarusИнститут микробиологии НАН БеларусиБеларусьInstitute of Microbiology of the National Academy of Sciences of BelarusBelarus201616112016047280Copyright © Киселева Е.П., Езубец А.П., Михайлопуло К.И., Новик Г.И., 20162016Киселева Е.П., Езубец А.П., Михайлопуло К.И., Новик Г.И.Kiseleva E., Ezybets A., Mikhailopulo K., Novik G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/255

Методами прямого и конкурентного иммуноферментного анализа проведено исследование динамики синтеза и распределения в системе клетка–среда антигенов 4 штаммов лактококков. Установлено, что антигены лактококков включают как компоненты клетки, локализованные в цитоплазме и клеточной стенке, так и секреторные соединения. Показано, что характер изменения концентрации антигенов лактококков в системе клетка–среда зависит от штамма, стадии роста культуры и состава среды. Результаты исследования могут найти применение при выделении компонентов клеток и секреторных продуктов лактококков для их последующего использования в биотехнологиях производства препаратов пробиотического действия.

Dynamics of synthesis and distribution in the system cell-environment of lactococcal antigens (4 strains) was investigated by direct and competitive ELISA. It was found that lactococcal antigens include intracellular, cell wall and secretory biopolymers. It was shown that lactococcal antigens are synthesized and distributed in the system cell-environment in dependence on strain, stage of culture growth and media composition. The data will find an application in biotechnology of probiotics.

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The authors declare that there are no conflicts of interest present.