vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-224Research ArticleСтатьиГЕНЕТИЧЕСКИЙ ПОЛИМОРФИЗМ И РАСПРОСТРАНЕННОСТЬ ВИРУСА МОЗАИКИ ЯБЛОНИ В САДАХ БЕЛАРУСИGENETIC POLYMORPHISM AND APPLE MOSAIC VIRUS PREVALENCE IN THE GARDENS OF BELARUSКУЗМИЦКАЯП. В.KUZMITSKAYAP. V.P.Kuzmitskaya@igc.byУРБАНОВИЧО. Ю.URBANOVICHO. Yu.O.Urbanovich@igc.byИнститут генетики и цитологии Н АН БеларусиInstitute of Genetics and Cytology of National Academy of Belarus201610112016031722Copyright © КУЗМИЦКАЯ П.В., УРБАНОВИЧ О.Ю., 20162016КУЗМИЦКАЯ П.В., УРБАНОВИЧ О.Ю.KUZMITSKAYA P.V., URBANOVICH O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/224

С помощью метода ОТ-ПЦР обнаружено, что среди деревьев из действующих промышленных и маточно-черенковых садов 1,5 % заражены вирусом мозаики яблони. У деревьев старше 50 лет инфекция не выявлена. Степень идентичности вирусов мозаики яблони, распространенных на территории Беларуси, определенная с помощью сиквенирования фрагментов гена, кодирующего белок оболочки, колебалась в пределах от 98,9 до 99,6 %. Анализ эволюционных взаимоотношений полученных нами нуклеотидных последовательностей и их гомологов из базы данных GenBank показал, что сходство между генотипами вируса наблюдается как у изолятов, выделенных из разных растений, растущих в одном географическом регионе, так и у изолятов, выделенных из одного вида растений, растущих в различных географических регионах. Это может быть следствием двух способов распространения вируса мозаики яблони – вегетативного и механического.

Apple trees of different cultivars growing in Belarus were tested using RT-PCR for Apple mosaic virus, an important economically and common pathogens in commercial orchards. Viral infected apple trees (1.5 %) were detected in the modern horticultural plantations and were not found among the old trees aged more than 50 years. The fragments of ApMV coat protein gene were cloned and sequenced. The analysis of their nucleotide sequences showed that the identity between them ranged from 98.9 to 99.6 %. The analysis of evolutionary relationships of nucleotide sequences obtained in this study and their homologs from the GenBank database revealed that the similarity between the viral genotypes was observed both between the isolates from various plants growing in the same geographical region, as well as between the isolates obtained from the same plant species, growing in different geographic regions. This picture was probably formed as a result of two methods of apple mosaic virus propagation: vegetative and mechanical.

вирус мозаики яблониApMVяблоняОТ-ПЦРфилогенетический анализгенкодирующий белок оболочкиApple mosaic virusApMVappleRT-PCRphylogenetic analysisсoat protein geneReferencesBereger, P. The complete nucleotide sequence of apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses / P. Bereger, P. Shiel // J. of Gen. Virol. – 2000. – Vol. 81. – P. 273–278.Bereger, P. The complete nucleotide sequence of apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses / P. Bereger, P. Shiel // J. of Gen. Virol. – 2000. – Vol. 81. – P. 273–278.Sanchez-Navarro, J. Nucleotide sequence of apple mosaic ilarvirus RNA 4 / J. Sanchez-Navarro, V. Pallas // J. of Gen. Virol. – 1994. – Vol. 75. – P. 1441–1445.Sanchez-Navarro, J. Nucleotide sequence of apple mosaic ilarvirus RNA 4 / J. Sanchez-Navarro, V. Pallas // J. of Gen. Virol. – 1994. – Vol. 75. – P. 1441–1445.Petrzik, K. Remarkable variability of apple mosaic virus capsid protein gene after nucleotide position 141 / K. Petrzik, O. Lenz // Arch. of Virol. – 2002. – Vol. 147, N 7. – P. 1275–1285.Petrzik, K. Remarkable variability of apple mosaic virus capsid protein gene after nucleotide position 141 / K. Petrzik, O. Lenz // Arch. of Virol. – 2002. – Vol. 147, N 7. – P. 1275–1285.Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica) / T. Thokchom [et al.] // Phytoparasitica. – 2009. – Vol. 37, N 4. – P. 375– 379.Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica) / T. Thokchom [et al.] // Phytoparasitica. – 2009. – Vol. 37, N 4. – P. 375– 379.Lichens – a new source or yet unknown host of herbaceous plant viruses? / K. Petrzik [et al.] // Eur. J. of Plant Pathol. – 2014. – Vol. 138, N 3. – P. 549–559.Lichens – a new source or yet unknown host of herbaceous plant viruses? / K. Petrzik [et al.] // Eur. J. of Plant Pathol. – 2014. – Vol. 138, N 3. – P. 549–559.Кухарчик, Н . В. Вирусные и фитоплазменные болезни плодовых и ягодных культур в Беларуси / Н. В. Кухарчик. – Минск: Беларус. навука, 2012. – 209 с.Кухарчик, Н . В. Вирусные и фитоплазменные болезни плодовых и ягодных культур в Беларуси / Н. В. Кухарчик. – Минск: Беларус. навука, 2012. – 209 с.Effect of apple mosaic virus on the growth, yield and quality of apple / B. B. Singh [et al.] // Punjab Hortic J. – 1979. – Vol. 19. – P. 80–82.Effect of apple mosaic virus on the growth, yield and quality of apple / B. B. Singh [et al.] // Punjab Hortic J. – 1979. – Vol. 19. – P. 80–82.Sutic, d. Virus diseases of fruit trees / D. Sutic, R. Ford, M. Tosic // Handbook of plant virus diseases. – 1999. – P. 321–432.Sutic, d. Virus diseases of fruit trees / D. Sutic, R. Ford, M. Tosic // Handbook of plant virus diseases. – 1999. – P. 321–432.Economic implications of a virus prevention program in deciduous tree fruits in the US / T. Cembali [et al.] // Crop Protection. – 2003. – Vol. 22, N 10. – P. 1149–1156.Economic implications of a virus prevention program in deciduous tree fruits in the US / T. Cembali [et al.] // Crop Protection. – 2003. – Vol. 22, N 10. – P. 1149–1156.Zawadzka, B. The effect of mosaic and rubbery wood infection on the growth and yield of apple trees / B. Zawadzka // Zesz. Probl. Postepow Nauk Roln. – 1983. – Vol. 291. – P. 385–390.Zawadzka, B. The effect of mosaic and rubbery wood infection on the growth and yield of apple trees / B. Zawadzka // Zesz. Probl. Postepow Nauk Roln. – 1983. – Vol. 291. – P. 385–390.Shiel, P. J. The complete nucleotide sequence of Apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses / P. J. Shiel, P. H. Berger // J. of Gen. Virol. – 2000. – Vol. 81. – P. 273–278.Shiel, P. J. The complete nucleotide sequence of Apple mosaic virus (ApMV) RNA 1 and RNA 2: ApMV is more closely related to alfalfa mosaic virus than to other ilarviruses / P. J. Shiel, P. H. Berger // J. of Gen. Virol. – 2000. – Vol. 81. – P. 273–278.Petrzik, K. Capsid protein sequence gene analysis of Apple mosaic virus infecting pears / K. Petrzik // Eur. J. of Plant Pathol. – 2005. – Vol. 111. – P. 355–360.Petrzik, K. Capsid protein sequence gene analysis of Apple mosaic virus infecting pears / K. Petrzik // Eur. J. of Plant Pathol. – 2005. – Vol. 111. – P. 355–360.EPPO Standards – Certification schemes – PM 4/27(1) Pathogen-tested material of Malus, Pyrus and Cydonia // EPPO Bull. – 1999. – Vol. 29, N 3. – P. 239–252.EPPO Standards – Certification schemes – PM 4/27(1) Pathogen-tested material of Malus, Pyrus and Cydonia // EPPO Bull. – 1999. – Vol. 29, N 3. – P. 239–252.Detection of four apple viruses by ELISA and RT-PCR assays in Turkey / K. Caglayan [et al.] // Turk. J. Agric. For. – 2006. – Vol. 30. – P. 241–246.Detection of four apple viruses by ELISA and RT-PCR assays in Turkey / K. Caglayan [et al.] // Turk. J. Agric. For. – 2006. – Vol. 30. – P. 241–246.Hassan, M. Simultaneous detection and identification of four pome fruit viruses by one- tube pentaplex RT-PCR / M. Hassan, A. Myrta, J. Polak // J. of Virol. Meth. – 2006. – Vol. 133. – P. 124–129.Hassan, M. Simultaneous detection and identification of four pome fruit viruses by one- tube pentaplex RT-PCR / M. Hassan, A. Myrta, J. Polak // J. of Virol. Meth. – 2006. – Vol. 133. – P. 124–129.Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control / W. Menzel, W. Jelkmann, E. Maiss // J. of Virol. Meth. – 2002. – Vol. 99. – P. 81–92.Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control / W. Menzel, W. Jelkmann, E. Maiss // J. of Virol. Meth. – 2002. – Vol. 99. – P. 81–92.Thompson, J. D. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice / J. D. Thompson, D. G. Higgins, T. J. Gibson // Nucl. Acids Res. – 1994. – Vol. 22, N 22. – P. 4673–4680.Thompson, J. D. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice / J. D. Thompson, D. G. Higgins, T. J. Gibson // Nucl. Acids Res. – 1994. – Vol. 22, N 22. – P. 4673–4680.MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0 / K. Tamura [et al.] // Mol. Biol. and Evolution. – 2013. – Vol. 30, N 12. – P. 2725–2729.MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0 / K. Tamura [et al.] // Mol. Biol. and Evolution. – 2013. – Vol. 30, N 12. – P. 2725–2729.Saitou, N. The neighbor-joining method: a new method for reconstructing phylogenetic trees / N. Saitou, M. Nei // Mol. Biol. and Evolution. – 1987. – Vol. 4, N 4. – P. 406–425.Saitou, N. The neighbor-joining method: a new method for reconstructing phylogenetic trees / N. Saitou, M. Nei // Mol. Biol. and Evolution. – 1987. – Vol. 4, N 4. – P. 406–425.Drake, J. W. Mutation rates among RNA viruses / J. W. Drake, J. J. Holland // Proc. Natl. Acad. Sci. USA. – 1999. – Vol. 96. – P. 13910–13913.Drake, J. W. Mutation rates among RNA viruses / J. W. Drake, J. J. Holland // Proc. Natl. Acad. Sci. USA. – 1999. – Vol. 96. – P. 13910–13913.Roossinck, M. J. Mechanisms of plant virus evolution / M. J. Roossinck // Annu. Rev. Phytopathol. – 1997. – Vol. 35. – P. 191–209.Roossinck, M. J. Mechanisms of plant virus evolution / M. J. Roossinck // Annu. Rev. Phytopathol. – 1997. – Vol. 35. – P. 191–209.

The authors declare that there are no conflicts of interest present.