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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestib</journal-id><journal-title-group><journal-title xml:lang="ru">Известия Национальной  академии наук Беларуси. Серия биологических наук</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the National Academy of Sciences of Belarus, Biological Series</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1029-8940</issn><issn pub-type="epub">2524-230X</issn><publisher><publisher-name>The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestib-186</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Химическая модификация полистирольных планшетов для ковалентной иммобилизации биомолекул</article-title><trans-title-group xml:lang="en"><trans-title>Chemical modification of polystyrene plates for covalent immobilization of biomolecules</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ткачев</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Tkachou</surname><given-names>S. V.</given-names></name></name-alternatives><email xlink:type="simple">pjc40@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фомина</surname><given-names>Е. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Fomina</surname><given-names>E. H.</given-names></name></name-alternatives><email xlink:type="simple">feg1@tut.by</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Владыко</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Vladiko</surname><given-names>A. S.</given-names></name></name-alternatives><email xlink:type="simple">vladyco@belriem.by</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Республиканский научно-практический центр эпидемиологии и микробиологии</institution></aff><aff xml:lang="en"><institution>The Republican Research and Practical Center for Epidemiology and Microbiology</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>07</day><month>06</month><year>2016</year></pub-date><volume>0</volume><issue>1</issue><fpage>38</fpage><lpage>44</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ткачев С.В., Фомина Е.Г., Владыко А.С., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Ткачев С.В., Фомина Е.Г., Владыко А.С.</copyright-holder><copyright-holder xml:lang="en">Tkachou S.V., Fomina E.H., Vladiko A.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestibio.belnauka.by/jour/article/view/186">https://vestibio.belnauka.by/jour/article/view/186</self-uri><abstract><p>- N,N-бис(карбоксиметил)лизином. Полученный таким образом активированный планшет после насыщения никелем специфически связывал на своей поверхности рекомбинантный 10 His-tags IgG.</p></abstract><trans-abstract xml:lang="en"><p>+ for the capture of the 10 His-tagged IgG.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>полистирольные планшеты</kwd><kwd>персульфат аммония</kwd><kwd>модификация поверхности</kwd><kwd>N</kwd><kwd>N-бис(карбоксиметил)лизин</kwd><kwd>никель</kwd><kwd>His-tag пептиды</kwd><kwd>иммуноферментный анализ</kwd><kwd>polystyrene microtiter plate</kwd><kwd>ammonium persulfate</kwd><kwd>surface modification N</kwd><kwd>N</kwd><kwd>N-bis(carboxymethyl)lysine</kwd><kwd>nickel</kwd><kwd>His-tag peptides</kwd><kwd>enzyme-linked immunosorbent assay</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Gray, J. J. The interaction of proteins with solid surfaces / J. J. Gray // Curr. Opin. Struct. Biol. - 2004. - Vol. 14, N 1. -P. 110-115.</mixed-citation><mixed-citation xml:lang="en">Gray, J. J. The interaction of proteins with solid surfaces / J. J. Gray // Curr. Opin. Struct. Biol. - 2004. - Vol. 14, N 1. -P. 110-115.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Butler, J. E. Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays / J. E. Butler // Methods. - 2000. - Vol. 22, N 1. - P. 4-23.</mixed-citation><mixed-citation xml:lang="en">Butler, J. E. Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays / J. E. Butler // Methods. - 2000. - Vol. 22, N 1. - P. 4-23.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">The immunochemistry of sandwich ELISAs-VI. Greater than 90 % of monoclonal and 75 % of polyclonal anti-fluorescyl capture antibodies (cabs) are denatured by passive adsorption / J. E. Butler [et al.] // Mol. Immunol. - 1993. - Vol. 30, N 13. -P. 1165-1175.</mixed-citation><mixed-citation xml:lang="en">The immunochemistry of sandwich ELISAs-VI. Greater than 90 % of monoclonal and 75 % of polyclonal anti-fluorescyl capture antibodies (cabs) are denatured by passive adsorption / J. E. Butler [et al.] // Mol. Immunol. - 1993. - Vol. 30, N 13. -P. 1165-1175.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Nonthermal plasma technology as a versatile strategy for polymeric biomaterials surface modification: a review / T. Desmet [et al.] // Biomacromolecules. - 2009. - Vol. 10, N 9. - P. 2351-2378.</mixed-citation><mixed-citation xml:lang="en">Nonthermal plasma technology as a versatile strategy for polymeric biomaterials surface modification: a review / T. Desmet [et al.] // Biomacromolecules. - 2009. - Vol. 10, N 9. - P. 2351-2378.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Novak, I. Study of surface properties of polyolefins modified by corona discharge plasma / I. Novak, V. Pollak, I. Chodak // Plasma Processes and Polym. - 2006. - Vol. 3, N 4-5. - P. 355-364.</mixed-citation><mixed-citation xml:lang="en">Novak, I. Study of surface properties of polyolefins modified by corona discharge plasma / I. Novak, V. Pollak, I. Chodak // Plasma Processes and Polym. - 2006. - Vol. 3, N 4-5. - P. 355-364.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Chemical modifications, mechanical properties and surface photo-oxidation of films of polystyrene / F. A. Bottino [et al.] // Polymer Testing. - 2004. - Vol. 23, N 4. - P. 405-411.</mixed-citation><mixed-citation xml:lang="en">Chemical modifications, mechanical properties and surface photo-oxidation of films of polystyrene / F. A. Bottino [et al.] // Polymer Testing. - 2004. - Vol. 23, N 4. - P. 405-411.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Goddard, J. M. Polymer surface modification for the attachment of bioactive compounds / J. M. Goddard, J. H. Hotchkiss // Prog. Polym. Sci. - 2007. - Vol. 32, N 7. - P. 698-725.</mixed-citation><mixed-citation xml:lang="en">Goddard, J. M. Polymer surface modification for the attachment of bioactive compounds / J. M. Goddard, J. H. Hotchkiss // Prog. Polym. Sci. - 2007. - Vol. 32, N 7. - P. 698-725.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Amination of polystyrene microwells: application to the covalent grafting of DNA probes for hybridization assays / N. Zammatteo [et al.] // Anal. Biochem. - 1996. - Vol. 236, N 1. - P. 85-94.</mixed-citation><mixed-citation xml:lang="en">Amination of polystyrene microwells: application to the covalent grafting of DNA probes for hybridization assays / N. Zammatteo [et al.] // Anal. Biochem. - 1996. - Vol. 236, N 1. - P. 85-94.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Strategies for direct attachment of hapten to a polystyrene support for applications in enzyme-linked immunosorbent assay (ELISA) / J. Kaur [et al.] // Analytica Chimica Acta. - 2004. - Vol. 506, N 2. - P. 133-135.</mixed-citation><mixed-citation xml:lang="en">Strategies for direct attachment of hapten to a polystyrene support for applications in enzyme-linked immunosorbent assay (ELISA) / J. Kaur [et al.] // Analytica Chimica Acta. - 2004. - Vol. 506, N 2. - P. 133-135.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Transparent polystyrene substrates with controllable surface chlorosulfonation: stable, versatile, and water-compatible precursors for functionalization / A. del Prado [et al.] // Macromolecules. - 2012. - Vol. 45, N 6. - P. 2648-2653.</mixed-citation><mixed-citation xml:lang="en">Transparent polystyrene substrates with controllable surface chlorosulfonation: stable, versatile, and water-compatible precursors for functionalization / A. del Prado [et al.] // Macromolecules. - 2012. - Vol. 45, N 6. - P. 2648-2653.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Bamfordt, C. H. Polymer surface functionalisation and grafting by a simple and inexpensive method / C. H. Bamfordt, K. G. AI-Lamee // Macromol. Rapid Commun. - 1994. - Vol. 15, N 4. - P. 379-384.</mixed-citation><mixed-citation xml:lang="en">Bamfordt, C. H. Polymer surface functionalisation and grafting by a simple and inexpensive method / C. H. Bamfordt, K. G. AI-Lamee // Macromol. Rapid Commun. - 1994. - Vol. 15, N 4. - P. 379-384.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Bamfordt, C. H. Studies in polymer surface functionalization and grafting for biomedical and other applications / C. H. Bamfordt, K. G. AI-Lamee // Polymer. - 1994. - Vol. 35, N 13. - P. 2844-2852.</mixed-citation><mixed-citation xml:lang="en">Bamfordt, C. H. Studies in polymer surface functionalization and grafting for biomedical and other applications / C. H. Bamfordt, K. G. AI-Lamee // Polymer. - 1994. - Vol. 35, N 13. - P. 2844-2852.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Киреев, В. В. Высокомолекулярные соединения / В. В. Киреев. - М.: Высш. школа, 1992. - 512 c.</mixed-citation><mixed-citation xml:lang="en">Киреев, В. В. Высокомолекулярные соединения / В. В. Киреев. - М.: Высш. школа, 1992. - 512 c.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Tiraferri, A. Direct quantification of negatively charged functional groups on membrane surfaces / A. Tiraferri, M. Elimelech // J. Membr. Sci. - 2012. - Vol. 389. - P. 499-508.</mixed-citation><mixed-citation xml:lang="en">Tiraferri, A. Direct quantification of negatively charged functional groups on membrane surfaces / A. Tiraferri, M. Elimelech // J. Membr. Sci. - 2012. - Vol. 389. - P. 499-508.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Modification of PS films by combined treatment of ozone aeration and UV irradiation in aqueous ammonia solution for the introduction of amine and amide groups on their surface / T. N. Murakami [et al.] // Appl. Surf. Sci. - 2005. - Vol. 249, N 1-4. - P. 425-432.</mixed-citation><mixed-citation xml:lang="en">Modification of PS films by combined treatment of ozone aeration and UV irradiation in aqueous ammonia solution for the introduction of amine and amide groups on their surface / T. N. Murakami [et al.] // Appl. Surf. Sci. - 2005. - Vol. 249, N 1-4. - P. 425-432.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Hermanson, G. T. Bioconjugate techniques / G. T. Hermanson. - 2nd ed. - Academic Press, Elsevier Inc., 2008. -1323 p.</mixed-citation><mixed-citation xml:lang="en">Hermanson, G. T. Bioconjugate techniques / G. T. Hermanson. - 2nd ed. - Academic Press, Elsevier Inc., 2008. -1323 p.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Hochuli, E. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues / E. Hochuli, H. Dobeli, A. Schacher // J. Chromatogr. - 1987. - Vol. 411. - P. 177-184.</mixed-citation><mixed-citation xml:lang="en">Hochuli, E. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues / E. Hochuli, H. Dobeli, A. Schacher // J. Chromatogr. - 1987. - Vol. 411. - P. 177-184.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Immobilized-metal affinity chromatography (IMAC): a review / H. Block [et al.] // Meth. Enzymol. - 2009. -Vol. 463. - P. 439-473.</mixed-citation><mixed-citation xml:lang="en">Immobilized-metal affinity chromatography (IMAC): a review / H. Block [et al.] // Meth. Enzymol. - 2009. -Vol. 463. - P. 439-473.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
