vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-166Research ArticleСтатьиСоздание рекомбинантного штамма Escherichia coli - продуцента диаденилатциклазы и ее использование для синтеза цикло-ди-АМФConstruction of Escherichia coli strain, producing di-adenylate cyclase and its application for cyclic di-AMP synthesisКазловскийИ. С.KazlovskijI. S.leonardo139@mail.ruРадевичД. С.RadevichD. S.noemail@neicon.ruРымкоА. Н.RymkoA. N.noemail@neicon.ruЩеколоваА. С.ShchokolovaA. S.noemail@neicon.ruКвачС. В.KvachS. V.noemail@neicon.ruЗинченкоА. И.ZinchenkoA. I.zinch@mbio.bas-net.byМеждународный государственный экологический университет им. А. Д. СахароваSakharov International State Ecological UniversityИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus201507062016045155Copyright © Казловский И.С., Радевич Д.С., Рымко А.Н., Щеколова А.С., Квач С.В., Зинченко А.И., 20162016Казловский И.С., Радевич Д.С., Рымко А.Н., Щеколова А.С., Квач С.В., Зинченко А.И.Kazlovskij I.S., Radevich D.S., Rymko A.N., Shchokolova A.S., Kvach S.V., Zinchenko A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/166

Сконструирован новый рекомбинантный штамм Escherichia coli pBtdac, продуцирующий диаденилатциклазу Bacillus thuringiensis. Количество диаденилатциклазы, продуцируемой этим штаммом, составляет 32 мг в 1 л культуральной жидкости. Изучена субстратная специфичность и стабильность полученного фермента. Продемонстрировано применение очищенной диаденилатциклазы для препаративного (600 мг) синтеза цикло-ди-АМФ.

A new recombinant strain of Escherichia coli pBtdac, producing diadenylate cyclase Bacillus thuringiensis has been constructed. The amount of the diadenylate cyclase produced by the strain is 32 mg per 1 L of culture broth. The substrate specificity and stability of the produced enzyme has been studied. Application of the purified diadenylate cyclase for preparative (600 mg) cyclic di-AMP synthesis has been demonstrated.

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The authors declare that there are no conflicts of interest present.