vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2026-71-3-219-226vestib-1019Research ArticleСтатьиФунгістатычнае ўздзеянне экстрактаў кары Frangula alnus Mill. і Rhamnus cathartica L. на некаторых ксілатрофных прадстаўнікоў аддзела BasidiomycotaFungistatic effect of bark extracts from Frangula alnus Mill. and Rhamnus cathartica L. on certain xylotrophic representatives of the phylum Basidiomycotahttps://orcid.org/0000-0002-5643-2254КаханоўскіА. І.KakhanouskiA. I.

Кохановский Александр Иванович – науч. сотрудник

ул. Симонова, 73, 212021, г. Могилёв

Aliaxandr I. Kakhanouski – Researcher

73, Simonova Str., 212021, Mogilev

akakhanouski@gmail.comhttps://orcid.org/0000-0002-9674-7648СпірыдовічА. У.SpirydovichA. V.

Спиридович Елена Владимировна – канд. биол. наук, доцент, заведующий лабораторией

ул. Сурганова, 2в, 220012, г. Минск

Alena V. Spirydovich – Ph. D. (Biol.), Associate Professor, Head of the Laboratory

2v, Surganov Str., 220012, Minsk

a.spirydovich@gmail.comhttps://orcid.org/0000-0002-7049-4080ШабашоваТ. Г.ShabashovaT. G.

Шабашова Татьяна Гарьевна – канд. биол. наук, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Tatiana G. Shabashova – Ph. D. (Biol.), Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

tiniti@inbox.ruГрупа навуковага суправаджэння фермерскай гаспадаркі «Сталетава»БеларусьGroup of the Agricultural Company “Staletava”BelarusЦэнтральны батанічны сад Нацыянальнай акадэміі навук БеларусіБеларусьCentral Botanical Garden of the National Academy of Sciences of BelarusBelarusІнстытут эксперыментальнай батанікі імя В. Ф. Купревіча Нацыянальнай акадэміі навук БеларусіБеларусьV. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of BelarusBelarus202604082026713219226Copyright © Каханоўскі А.І., Спірыдовіч А.У., Шабашова Т.Г., 20262026Каханоўскі А.І., Спірыдовіч А.У., Шабашова Т.Г.Kakhanouski A.I., Spirydovich A.V., Shabashova T.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/1019

Данное исследование посвящено изучению фунгистатического эффекта экстрактов коры Frangula alnus Mill. и Rhamnus cathartica L. на представителей отдела Basidiomycota – фитопатогенов и ксилотрофов, вызывающих повреждение лесных культур и деревянных конструкций. В эксперименте было исследовано шесть штаммов грибов из Белорусской коллекции непатогенных микроорганизмов Института микробиологии НАН Беларуси: Phellinus igniarius (BIM F-205), Phellinus tremulae (BIM F-204), Fomitopsis pinicola (BIM F-225), Phellinus pini (BIM F-321), Gloeophyllum sepiarium (BIM F-227), Serpula lacrymans (BIM F-835). Результаты показали, что экстракт F. alnus, содержащий вторичные метаболиты – гидроксиантрахиноны, значительно подавлял рост пяти из шести видов, при этом наиболее сильный эффект наблюдался для S. lacrymans (57 % подавления). Дополнительный анализ с использованием экстрактов с различными концентрациями гидроксиантрахинонов выявил четкую дозозависимую реакцию G. sepiarium, что подтверждает ключевую роль этих соединений в фунгистатическом эффекте. Результаты исследования расширяют понимание роли гидроксиантрахинонов в фитоиммунитете и открывают перспективы их применения в биотехнологии лесных культур.

The study examines the fungistatic effect of bark extracts from Frangula alnus Mill. and Rhamnus cathartica L. on representatives of the phylum Basidiomycota – phytopathogens and xylotrophs that cause damage to forest crops and wooden structures. Six fungal strains from the Belarus Collection of Non-pathogenic Microorganisms of the Institute of Microbiology of the NAS of Belarus were investigated in the experiment: Phellinus igniarius (BIM F-205), Phellinus tremulae (BIM F-204), Fomitopsis pinicola (BIM F-225), Phellinus pini (BIM F-321), Gloeophyllum sepiarium (BIM F-227), and Serpula lacry­ mans (BIM F-835). The results showed that the F. alnus extract, containing hydroxyanthraquinone secondary metabolites, signi ficantly suppressed the growth of five out of the six species, with the strongest effect observed for S. lacrymans (57 % inhibition). Further analysis using extracts with varying concentrations of hydroxyanthraquinones revealed a clear dose-dependent response in G. sepiarium, confirming the key role of these compounds in the fungistatic effect. The findings expand the understanding of the role of hydroxyanthraquinones in phytoimmunity and open prospects for their application in forest crop biotechnology.

Frangula alnusRhamnus catharticaгидроксиантрахиноныфунгистатический эффектBasidiomycotaфитопатогеныксилотрофыFrangula alnusRhamnus catharticahydroxyanthraquinonesfungistatic effectBasidiomycotaphytopathogensxylotrophsReferencesКаханоўскі, А. І. Тканкавая спецыфічнасць, лакалізацыя і мадыфікацыя гідроксіантрахінонаў у прадстаўнікоў сямейства Крушынавыя (Rhamnaceae Juss.) / А. І. Каханоўскі, А. У. Спірыдовіч // Ботаника (исследования): сб. науч. тр. / НАН Беларуси [и др.]; науч. ред. Н. А. Ламан. – Мн., 2025. – Вып. 56. – С. 206–213.Kakhanouski A. I., Spіrydovіch A. V. Tissue specificity, localization and modification of hydroxyanthraquinones in representatives of the buckthorn family (Rhamnaceae Juss.). Botanika (issledovaniya): sborniknauchnykhtrudov [Botany (research): collection of scientific papers]. Minsk, 2025, iss. 56, pp. 206–213 (in Belarussian).Кохановский, А. И. Некоторые аспекты биологического действия эмодина на растения / А. И. Кохановский, В. М. Юрин, Е. Ю. Кохановская // Журнал Белорусского государственного университета. Биология. – 2021. – Т. 2. – С. 97–103.Kokhanovskii A. I., Yurin V. M., Kokhanovskaya E. Yu. Some aspects of biological effect of emodin on plants. Zhurnal Belorusskogo gosudarstvennogo universiteta. Biologiya = Journal of the Belarusian State University. Biology, 2021, no. 2, pp. 97–103 (in Russian).A systematic review of pharmacological potential of phytochemicals from Rumexabyssinicus Jacq. / A. Ntemafack, M. Ayoub, Q. P. Hassan, S. G. Gandhi // South African Journal of Botany. – 2023. – Vol. 154. – P. 11–25. https://doi.org/10.1016/j.sajb.2023.01.013Ntemafack A., Ayoub M., Hassan Q. P., Gandhi S. G. A systematic review of pharmacological potential of phytochemicals from Rumex abyssinicus Jacq. South African Journal of Botany, 2023, vol. 154, pp. 11–25. https://doi.org/10.1016/j.sajb.2023.01.013Membrane-related effects underlying the biological activity of the anthraquinones emodin and barbaloin / D. S. Alves, L. Pérez-Fons, A. Estepa, V. Micol // Biochemical Pharmacology. – 2004. – Vol. 68, N 3. – P. 549–561. https://doi.org/10.1016/j.bcp.2004.04.012Alves D. S., Pérez-Fons L., Estepa A., Micol V. Membrane-related effects underlying the biological activity of the anthraquinones emodin and barbaloin. Biochemical Pharmacology, 2004, vol. 68, no. 3, pp. 549–561. https://doi.org/10.1016/j.bcp.2004.04.012Anthraquinones Isolated from Cassiatora (Leguminosae) Seed Show an Antifungal Property against Phytopathogenic Fungi / Y.-M. Kim, Ch.-H. Lee, H.-G. Kim, H.-S. Lee // Journal of Agricultural and Food Chemistry. – 2004. – Vol. 52, N 20. – P. 6096–6100. https://doi.org/10.1021/jf049379pKim Y.-M., Lee Ch.-H., Kim H.-G., Lee H.-S. Anthraquinones Isolated from Cassiatora (Leguminosae) Seed Show an Antifungal Property against Phytopathogenic Fungi. Journal of Agricultural and Food Chemistry, 2004, vol. 52, no. 20, pp. 6096–6100. https://doi.org/10.1021/jf049379pBiodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lig- nin / A. T. Mar tínez, M. Speranza, F. J. Ruiz-Dueñas [et al.] // International Microbiology. – 2005. – Vol. 8, N 3. – P. 195–204.Martínez A. T., Speranza M., Ruiz-Dueñas, F. J., Ferreira P., Camarero S., Guillén F., Martínez M. J., Gutiérrez A., del Río J. C. Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. International Microbiology, 2005, vol. 8, no. 3, pp. 195–204.Hydrophobins in the Life Cycle of the Ectomycorrhizal Basidiomycete Tricholomavaccinum / D. Sammer, K. Krause, M. Gube [et al.] // PLoS ONE. – 2016. – Vol. 11, N 12. – Art. e0167773. https://doi.org/10.1371/journal.pone.0167773Sammer D., Krause K., Gube M., Wagner K., Kothe E. Hydrophobins in the Life Cycle of the Ectomycorrhizal Basidiomycete Tricholomavaccinum. PLoS ONE, 2016, vol. 11, no. 12, art. e0167773. https://doi.org/10.1371/journal.pone.0167773Pterostilbene production of tomato transformed with resveratrol synthase and resveratrol O-methyltransferase genes / Y. Zhang, L. Ni, Sh. Chen [et al.] // Plant Science. – 2022. – Vol. 322. – Art. 111343. https://doi.org/10.1016/j.plantsci.2022.111343Zhang Y., Ni L., Chen Sh., Qin Y., Ding X., Li J., Pan Y., Zhang X. Pterostilbene production of tomato transformed with resveratrol synthase and resveratrol O-methyltransferase genes. Plant Science, 2022, vol. 322, art. 111343. https:// doi.org/10.1016/j.plantsci.2022.111343Transgenic Rice Expressing Isoflavone Synthase Gene from Soybean Shows Resistance Against Blast Fungus (Magnaportheoryzae) / S. Pokhrel, S. K. Ponniah, Y. Jia [et al.] // Plant Disease. – 2021. – Vol. 105, N 10. – P. 3141–3146. https://doi.org/10.1094/pdis-08-20-1777-rePokhrel S., Ponniah S. K., Jia Y., Yu O., Manoharan M. Transgenic Rice Expressing Isoflavone Synthase Gene from Soybean Shows Resistance Against Blast Fungus (Magnaportheoryzae). Plant Disease, 2021, vol. 105, no. 10, pp. 3141–3146. https://doi.org/10.1094/pdis-08-20-1777-reTransgenic Tobacco and Arabidopsis Plants Expressing the Two Multifunctional Sorghum Cytochrome P450 Enzymes, CYP79A1 and CYP71E1, Are Cyanogenic and Accumulate Metabolites Derived from Intermediates in Dhurrin Biosynthesis / S. Bak, C. E. Olsen, B. A. Halkier, B. L. Møller // Plant Physiology. – 2000. – Vol. 123, N 4. – P. 1437–1448. https://doi.org/10.1104/pp.123.4.1437Bak S., Olsen C. E., Halkier B. A., Møller B. L. Transgenic Tobacco and Arabidopsis Plants Expressing the Two Multifunctional Sorghum Cytochrome P450 Enzymes, CYP79A1 and CYP71E1, Are Cyanogenic and Accumulate Metabolites Derived from Intermediates in Dhurrin Biosynthesis. Plant Physiology, 2000, vol. 123, no. 4, pp. 1437–1448. doi:10.1104/ pp.123.4.1437. PMID: 10938360. PMCID: PMC59100.Octaketide Synthase from Polygonum cuspidatum Implements Emodin Biosynthesis in Arabidopsis thaliana / Y. Guo, S. Nassar, L. Ma [et al.] // Plant and Cell Physiology. – 2021. – Vol. 62, N 3. – P. 424–435. https://doi.org/10.1093/pcp/pcaa135Guo Y., Nassar S., Ma L., Feng G., Li X., Chen M., Chai T., Abdel-Rahman I. A. M., Beuerle T., Beerhues L., Wang H., Liu B. Octaketide Synthase from Polygonum cuspidatum Implements Emodin Biosynthesis in Arabidopsis thaliana. Plant and Cell Physiology, 2021, vol. 62, no. 3, pp. 424–435. https://doi.org/10.1093/pcp/pcaa135

The authors declare that there are no conflicts of interest present.